The chromokinesin Klp3a is required for segregating acentrics. (A) Still images from a time-lapse movie of a control neuroblast (I-CreI alone) showing sister acentrics (red) separating and moving to opposite poles while in close association with microtubules (green). (B) Still images from a time-lapse movie of a neuroblast from a female Df klp3a/klp3a1124 with I-CreI–induced acentrics (Video 7). In neuroblasts depleted of Klp3a, acentric sisters remain fused by a thin stretch of chromatin (dashed arrow) with each other or with the opposite daughter nucleus and segregate to the same pole. Bars, 2 µm. Time in seconds. (C and D) Bar graphs showing the percentage of control and klp3a-defective neuroblasts with sister acentric fusions and the percentage of control and klp3a-defective neuroblasts with unequal acentric segregation.
