Acentrics travel poleward in anaphase while highly associated with microtubules. Microtubules are in green and chromosomes in red. (A) Images from a time-lapse movie of a control neuroblast from metaphase (0 s) through telophase (569 s). (B) Still images from a time-lapse movie of a mitotic neuroblast with I-CreI–induced acentrics (Video 3). Acentrics are positioned to the edge of the metaphase plate while in contact with microtubules (arrows and arrowheads in merge panel at 0 s). Sister acentrics (arrowheads in H2Av-RFP panels) are held together on the cell equator after the intact sisters have separated (0 to 98 s). Sister acentrics eventually separate and move toward opposite poles while associated with microtubules. In all 47 cells imaged, the acentrics were strongly associated with microtubules. (C) Line graphs from a compilation of seven control videos showing the relative fluorescence intensities in arbitrary units (AU) of microtubules (green line) and chromosomes (red) calculated between the yellow dashed lines at the time points 82, 116, 157, 199, and 267 s after anaphase. (D) Line graphs from a compilation of seven videos of I-CreI–expressing neuroblasts showing the relative fluorescence intensities of microtubules (green line) and chromosomes (red) calculated between the yellow dashed lines at time points 98, 126, 168, 189, and 266 s after anaphase. Bars, 2 µm. Time in seconds. Error bars represent SDs of the fluorescent intensities at all points tested. (E) 3D rendering of a video of a neuroblast division with I-CreI induced acentrics (Video 4). Bar, 2 µm. The 3D rendering from a 180° rotation from multiple images taken at the same time point (500 s) show the association of acentrics with microtubules (arrowheads). Similar 3D rendering were generated from a total of seven videos.