Acentrics migrate toward the spindle pole during anaphase with their telomeres leading or lagging. Chromosomes labeled with H2Av-RFP (red) and the telomere protein HOAP labeled with GFP (green). (A) Images from a time-lapse movie of a control mitotic neuroblast. (B) Still images from a time-lapse movie of a mitotic neuroblast with I-CreI–induced acentrics (Video 2). Acentrics lag on the spindle equator (0–106 s) but eventually separate (211 s) and move poleward with their telomere either facing the spindle equator (arrowhead) or spindle pole (arrow). Bars, 2 µm. Time in seconds. (C) Percentages of acentrics that migrate with telomeres leading (n = 62) or lagging (n = 82).