Microtubule channels remain open and kinetochore disassembly is delayed in response to error. (A) Spindles stained for DNA (blue), tubulin (green), and AIR-2 (red). When AIR-2 remains in rings (row 4, capg-1(RNAi) shown), the spindle is significantly wider than when AIR-2 is microtubule associated (rows 1–3); spindle widths are denoted within each image. (B) Box plot shows the widths of spindles in which AIR-2 is relocalized compared with those in which AIR-2 remains in rings. Spindles measured had chromosome distances between 2.7 µm and 4.2 µm. Box represents first quartile, median, and third quartile. Lines extend to data points within 1.5 interquartile range. N.S., not significant; asterisk represents significant difference (two-tailed t test; *, P < 0.001). (C) Spindles stained for DNA (blue), tubulin (green, columns 1 and 5), AIR-2 (green, column 4) and KNL-3 (red). KNL-3 remains chromosome-associated when AIR-2 remains in rings; 30°C treatment shown. Bars, 2.5 µm.