Summary of modeling. (A) Cartoon model of Zwilch (PDB ID: 3IF8). (B) Models of ROD were generated by a structure prediction program (I-TASSER). (C) Structures of the yeast homologue of ZW10 (Dsl1p), the N terminus (37–355; PDB ID: 3ETU), and the C terminus (333–684; PDB ID: 3K8P), were used for ZW10. NRH, NAG–ROD homology. (D) Typical negative-stain class averages of ZW10 obtained from ISAC. The averages highlight the inherent flexibility in the sample. Bar, 10 nm.