Single-site mutations within TPX2 α5–α7 do not interfere with the interaction of γ-TuRC. Immunoprecipitation of 1 µM GFP-tagged TPX2 constructs from Xenopus egg extracts using an antibody specific against GFP. The same GFP antibody was used for detection in the immunoblot, along with commercial antibodies against GCP5 and γ-tubulin. Assay was repeated three times. WT, wild type.