Figure 2. Multiple domains of TPX2... | Rockefeller University Press

Figure 2.

$Figure 2. Multiple domains of TPX2 contribute to its ability to bind and bundle MTs in vitro. (A) Co-sedimentation assay of 1 µM of various TPX2 constructs with Taxol-stabilized MTs in vitro. The ratio of protein in the supernatant and pellet after centrifugation was used to calculate the fraction of total protein bound to MTs. Values are the mean of two experiments, and error bars represent standard deviation. *, TPX2 α6 was only tested once. (B) MT bundling assay using Alexa Fluor 568–labeled GMPCPP-stabilized MTs in vitro with 1 µM of various TPX2 constructs. Bar, 10 µm. Assay was repeated three times.$

Multiple domains of TPX2 contribute to its ability to bind and bundle MTs in vitro. (A) Co-sedimentation assay of 1 µM of various TPX2 constructs with Taxol-stabilized MTs in vitro. The ratio of protein in the supernatant and pellet after centrifugation was used to calculate the fraction of total protein bound to MTs. Values are the mean of two experiments, and error bars represent standard deviation. *, TPX2 α6 was only tested once. (B) MT bundling assay using Alexa Fluor 568–labeled GMPCPP-stabilized MTs in vitro with 1 µM of various TPX2 constructs. Bar, 10 µm. Assay was repeated three times.