Figure 3. ACBD5 and VAPB mediate PO–ER... | Rockefeller University Press

Figure 3.

$Figure 3. ACBD5 and VAPB mediate PO–ER association. (A) Representative electron micrographs of PO–ER associations in COS-7 cells transfected with control vector, ACBD5, VAPB, and ACBD5 + VAPB (arrowheads highlight close ER–PO association). Electron-dense material between the PO–ER membranes is visible (see arrows in enlargement). (B) Quantitative analysis of the mean fraction of POs associated with the ER. (C) Assessment of the mean PO membrane surface in direct contact with the ER membrane. (D) Representative electron micrographs of PO–ER associations in HepG2 cells treated with control, ACBD5, VAPB, and ACBD5 + VAPB siRNAs. Arrowheads in overview mark POs with limited or no ER contact. (E) Quantitative analysis of mean fraction of POs associated with the ER after siRNA treatments. (F) Assessment of mean PO–ER membrane contact after siRNA treatment. (G) Immunoblot showing ACBD5 and VAPB signals after silencing with correspondent siRNAs. GAPDH, loading control. Data were analyzed by one-way analysis of variance with Tukey’s multiple comparison test; ns, not significant; **, P ≤ 0.01; ***, P ≤ 0.001. Error bars represent SEM, with three to six experiments per condition. Bars: (main) 200 nm; (zoom) 50 nm. M, mitochondrion; N, nucleus.$

ACBD5 and VAPB mediate PO–ER association. (A) Representative electron micrographs of PO–ER associations in COS-7 cells transfected with control vector, ACBD5, VAPB, and ACBD5 + VAPB (arrowheads highlight close ER–PO association). Electron-dense material between the PO–ER membranes is visible (see arrows in enlargement). (B) Quantitative analysis of the mean fraction of POs associated with the ER. (C) Assessment of the mean PO membrane surface in direct contact with the ER membrane. (D) Representative electron micrographs of PO–ER associations in HepG2 cells treated with control, ACBD5, VAPB, and ACBD5 + VAPB siRNAs. Arrowheads in overview mark POs with limited or no ER contact. (E) Quantitative analysis of mean fraction of POs associated with the ER after siRNA treatments. (F) Assessment of mean PO–ER membrane contact after siRNA treatment. (G) Immunoblot showing ACBD5 and VAPB signals after silencing with correspondent siRNAs. GAPDH, loading control. Data were analyzed by one-way analysis of variance with Tukey’s multiple comparison test; ns, not significant; **, P ≤ 0.01; ***, P ≤ 0.001. Error bars represent SEM, with three to six experiments per condition. Bars: (main) 200 nm; (zoom) 50 nm. M, mitochondrion; N, nucleus.