FKB-6 localizes to the cytoplasmic space throughout the germline. (A) Western blot of V5 antigen (top) and β-tubulin (bottom) as loading control of the genotypes indicated. (B) Projections of a z-stack halfway through the nuclei of V5::fkb-6 transgenic worms. Gonad stained with SYP-1 (green) and DAPI (red). (C) Representative images of V5 antibody staining in V5::fkb-6 strain in PMT, TZ, EP, MP, and LP. FKB-6 (via V5; green) and DAPI (red). Images on the bottom are V5::FKB-6 channel (white) of the images shown on the left. (D–H) Early pachytene images of V5::fkb-6 strain stained for V5 (red); in green, GFP::LEM-2 (D), GFP::PGL-1 (E), DHC-1::GFP (F), ZYG-12::GFP (G), and agglutinin (H). (I and J) Intensity plots of the LEM-2::GFP (I) or agglutinin (H) and V5::fkb-6 strain. Shaded boxes are part of the adjacent nucleus. Bars, 2 µm.