fkb-6 mutants accumulate recombination intermediates. (A) Representative images of RAD-51 antibody staining in wild-type and fkb-6 mutants in PMT/zone 1, TZ/zone 3, EP/zone 4, and LP/zone 7. Stained with RAD-51 (green) and DAPI (red). Images are a z-stack projection halfway through the nuclei. Bars, 2 µm. (B) Quantification of RAD-51 immunostaining. Representation of zones in terms of meiotic stage are as indicated in Fig. 1 E. Error bars are SEM. n nuclei: wild type = 718 and fkb-6 = 610. (C) Apoptotic nuclei per gonadal arm in the genotypes indicated. Error bars are SEM. n gonads: wild type = 125, fkb-6 = 74, syp3 = 57, spo-11 = 42, fkb-6;spo-11 = 56, ced-4 = 22, and fkb-6;ced-4 = 21. (D) Distribution of the number of COSA-1 foci per gonad in LP nuclei. n nuclei: wild type = 168 and fkb-6 = 123.