Figure 4.
CKA and Strip are in a complex with dynein. (A and B) Immunoprecipitation of the dynein intermediate chain (DIC; A) or the dynein heavy chain (DHC; B) from cultured Drosophila cells expressing GFP or GFP-CKA demonstrated that GFP-CKA, but not GFP alone, coimmunoprecipitated with dynein. (C) Immunoprecipitation of the dynein intermediate chain from cultured Drosophila cells also coimmunoprecipitated endogenous Strip protein. Lysates were divided in half and put onto beads in the presence or absence (controls) of antibody. Representative blots are shown. IB, immunoblot; IP, immunoprecipitate. A and C, n > 3 repeats; B, n = 2 repeats.