CKA modulates autophagosome transport. (A) Few autophagosome punctae were found in terminal boutons of control animal synaptic terminals, whereas autophagosomes accumulated in terminal boutons of animals depleted of CKA specifically in motoneurons by RNAi expression. Bar, 5 µm. (B) Quantification of autophagic vesicle (AV) accumulation in terminal boutons of NMJ4 for controls (dicer2 and a C911 sequence-specific off-target) and CKA loss of function. n, number of terminal boutons. Berger’s unconditional exact test results: *, P < 0.05; **, P < 0.005; ns, not significant. (C) A Western blot of larval brains from Cka² homozygotes, CKA-depleted animals (elav-Gal4>Cka-RNAi-A, UAS-dicer2), and controls (elav-Gal4>dicer2). The graph shows the ratio of CKA to α-tubulin in Cka² homozygotes and CKA-depleted animals normalized to controls set at 1.0. Data represent the mean and SEM of three separate experiments. IB, immunoblot. Paired, one-tailed t test results: *, P < 0.05; **, P < 0.005; ***, P ≤ 0.0001.