DOCK7/p116Rip signaling controls somal translocation. (A–F) Neuroblasts dissociated from the V-SVZ of P1–3 mice were coelectroporated with one of the indicated constructs and a plasmid coexpressing EGFP and PACT-mKO1, a marker for the centrosome. Cells were reaggregated, embedded in Matrigel, and subjected to confocal live-cell imaging. (A) Examples of time-lapse series showing centrosome and cell soma movements during migration in neuroblasts transfected with indicated constructs. Images are taken from Videos 8–12. Signals of EGFP and PACT-mKO1 are shown in green and red, respectively. White dashed lines indicate advancement of the rear edge of each cell. Arrowheads indicate centrosome position. Bars, 10 µm. (B and C) Temporal changes in the distance between the nucleus and the centrosome (N–C distance; B) and velocity of the cell body (green lines) and the centrosome (red lines; C) in migrating neuroblasts expressing indicated constructs. (D–F) Quantification of velocity (D), somal translocation events (E), and maximum nucleus–centrosome distance (F) for neuroblasts expressing indicated constructs. n = 6 cells (scr#2); n = 5 cells (p116Rip#1); n = 7 cells (Dock7#2); n = 12 cells (Dock7#2 + DOCK7); and n = 8 cells (Dock7#2 + DOCK7Δp116Rip); from 5–12 animals for each condition. Data are shown as means ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, P > 0.05; one-way ANOVA with Dunnett’s post hoc test.
