Knockdown of DOCK7 affects the migration distance, displacement, speed, and LP branching frequency of migrating neuroblasts. (A–F) P2–3 mouse pups were electroporated with plasmids expressing tdTomato and scr#1, Dock7#2 shRNA, or the rescue construct (Dock7#2 + DOCK7). After 5 d, acute sagittal brain slices were prepared, and tdTomato⁺ transfected neuroblasts were imaged by spinning-disk confocal microscopy over a 4-h time period. (A) Time-lapse sequence of scr#1 or Dock7#2 shRNA-expressing cells migrating in the lower vertical arm of the RMSp. Five cells in each slice are labeled in the 0-min panel, and their tracks over time are indicated by lines of the same color. Bars, 70 µm. (B–D) Quantifications of the mean migrated distance (B), displacement (C), and velocity (D) of neuroblasts expressing indicated constructs. n = 50–61 cells from five to seven animals for each condition. (E) Examples of time-lapse series of scr#1 or Dock7#2 shRNA-expressing cells in the lower vertical arm of the RMSp (higher magnification). Arrowheads indicate branching of LP. Bars, 50 µm. (F) Quantification of the number of branching events per h for neuroblasts expressing indicated constructs. n = 50–61 cells from five to seven animals for each condition. Data are shown as means ± SEM. **, P < 0.01; ****, P < 0.0001; ns, P > 0.05 compared with scr#1; one-way ANOVA with Dunnett’s post hoc test.