DOCK7 function is required for the migration of neuroblasts along the RMS. (A) Schematic drawing of postnatal in vivo electroporation. A virtual line (red) connecting the right eye to the craniometrical landmark λ serves as positional marker for plasmid injection into the lateral ventricle (LV). Lateral bars indicate position of electrodes. (B) Composite confocal images of forebrains of mice electroporated at P3 with plasmids expressing EGFP and nontargeting shRNA (scr#1), Dock7-targeting shRNAs (Dock7#1 and Dock7#2), or Dock7#2 shRNA together with DOCK7 cDNA (Dock7#2 + DOCK7) and sacrificed at P10 showing distribution of EGFP⁺ transfected cells along the V-SVZ–RMS–OB pathway. Slices were counterstained with DAPI. Dotted lines outline borders of sagittal slices. Bar, 500 µm. (C) Quantification of the distribution of EGFP⁺ transfected cells along the V-SVZ–RMS–OB pathway in the forebrains of mice electroporated at P3 and sacrificed at P10. n = 1,316–2,511 cells from at least three animals for each condition. (D) Quantification of the distribution of EGFP⁺ transfected cells along the V-SVZ–RMS–OB pathway in the forebrains of mice electroporated at P3 and sacrificed at P17. n = 2,379–2,462 cells from at least three animals for each condition. (E) Enlarged images of neuroblasts in RMSp regions of mice electroporated with indicated constructs at P3 and sacrificed at P10. Arrowheads indicate branching of LP. Bar, 20 µm. (F) Quantification of the length of the LP of neuroblasts in the RMS of mice electroporated with indicated constructs at P3 and sacrificed at P10. n = 91–680 cells from at least three animals for each condition. Data are shown as means ± SEM. **, P < 0.01; ***, P < 0.001; ns, P > 0.05 compared with scr#1; one-way ANOVA with Dunnett’s post hoc test (C and F) or Student’s t test (D).