RhoGEF2, but not AnxB9, accumulation at cell wounds is actin-dependent. (A–E) Confocal xy projection images from Drosophila NC4–6 staged embryos coexpressing sfGFP-RhoGEF2 and sChMCA injected with injection buffer + 50% DMSO (control; A–A″), C3 exoenzyme (Rho1 inhibitor; B–B″), Y-27632 (Rok inhibitor; C–C″), LatB (depolymerizes F-actin; D–D″), and phalloidin (stabilizes F-actin; E–E″). (F–F″) Two confocal xy projection images at ∼3-s and 45-s time points from Drosophila NC4–6 staged embryos coexpressing eGFP-AnxB9 and sK2MCA. The fastest acquirable time point is labeled ∼3 s (G) xy Kymograph across the wound area from Drosophila NC4–6 staged embryos coexpressing eGFP-AnxB9 and sK2MCA. (H and I) Confocal xy projection images from Drosophila NC4–6 staged embryos expressing eGFP-AnxB9 (H) or sfGFP-RhoGEF2 (I) at three time points (5, 10, and 15 s). (J–J″) Confocal xy projection images from Drosophila NC4–6 staged embryos coexpressing eGFP-AnxB9 and sK2MCA that have been injected with LatB. Time after wounding is indicated. Bars, 20 µm.