RhoGEF2, Pbl, and RhoGEF3 interact with Rho1, Cdc42, and Rac1, respectively, during cell wound repair. (A–I″) Confocal xy projection images at 180 s after wounding from Drosophila NC4–6 staged embryos coexpressing sfGFP-RhoGEF2 (A–C″), Pbl-eGFP (D–F″), or sfGFP-RhoGEF3 (G–I″) with ChFP-Rho1 (A–A″, D–D″, and G–G″), ChFP-Rac1 (B–B″, E–E″, and H–H″), or ChFP-Cdc42 (C–C″, F–F″, and I–I″). (J–R) Smoothened fluorescence (Fluor.) intensity (arbitrary units) profiles derived from averaged fluorescence intensity values over a 10-pixel width across the wound area in the embryo shown (A″–I″). Error bars represent the 95% confidence interval. (S) GST pulldown assays with ³⁵S-labeled in vitro translated RhoGEF2, Pbl, and RhoGEF3. The GST-Rho1, Rac1, and Cdc42 proteins were loaded with GDP or GTP as indicated. Time after wounding is indicated. Bars, 20 µm.