Figure 4. Dynamics of the apicosome in... | Rockefeller University Press

Figure 4.

$Figure 4. Dynamics of the apicosome in mitotic and postmitotic isolated cells. (A and B) Live imaging of mitotic and postmitotic H9 cells expressing EZRIN–GFP (A) or PODXL–GFP (B). Time stamp, h:min. Although most PODXL–GFP is segregated to one daughter cell (top), a fraction of the PODXL–GFP material is occasionally seen in the other daughter cell (bottom), revealing an unequal segregation of PODXL. (C–G) Confocal images of mitotic H9 cells (C and D) and two-cell H9 clones (E–G) stained with indicated markers. (G-i) Magnified image of the boxed region in G, revealing the presence of two primary cilia within a mature lumen shared by two cells. Bars: (A and B) 25 µm; (C–G) 10 µm; (G-i) 3 µm. For all images, blue indicates DNA staining (HOECHST).$

Dynamics of the apicosome in mitotic and postmitotic isolated cells. (A and B) Live imaging of mitotic and postmitotic H9 cells expressing EZRIN–GFP (A) or PODXL–GFP (B). Time stamp, h:min. Although most PODXL–GFP is segregated to one daughter cell (top), a fraction of the PODXL–GFP material is occasionally seen in the other daughter cell (bottom), revealing an unequal segregation of PODXL. (C–G) Confocal images of mitotic H9 cells (C and D) and two-cell H9 clones (E–G) stained with indicated markers. (G-i) Magnified image of the boxed region in G, revealing the presence of two primary cilia within a mature lumen shared by two cells. Bars: (A and B) 25 µm; (C–G) 10 µm; (G-i) 3 µm. For all images, blue indicates DNA staining (HOECHST).