The TPR domain of UNC-45a contributes to the assembly of contractile actomyosin bundles. (A) Representative examples of untransfected WT cells and UNC-45a knockout (KO) cells transfected with various UNC-45a–GFP constructs. F-actin and focal adhesions were visualized by phalloidin and by an antibody against vinculin, respectively. The borders of GFP-positive “rescue cells” are highlighted with yellow lines. Bars, 20 µm. (B) The length distributions of vinculin-positive focal adhesions. The number of focal adhesions in each size group was divided by the total focal adhesion number of the same cell. WT, n = 1,600 focal adhesions from 25 cells; UNC-45a knockout, n = 1,363 focal adhesions from 21 cells; UNC-45a–GFP, n = 1,054 focal adhesions from 20 cells; ΔTPR-GFP, n = 1,800 focal adhesions from 30 cells; ΔUCS-GFP, n = 1,341 focal adhesions from 22 cells. Data are represented as mean ± SEM (Fig. S5 D).