UNC-45a depletion impairs stress fiber assembly and focal adhesion maturation. (A) Visualization of actin filaments, endogenous UNC-45a, and NM-IIA heavy chains in WT and UNC-45a knockout (KO) cells (cultured on noncoated coverslips). (B) Co-cultivated WT and UNC-45a knockout cells stained for F-actin (phalloidin), UNC-45a, NM-IIA heavy chains, and vinculin. Yellow outlines indicate the borders of UNC-45a knockout cells. Bars, 20 µm.