Chemically induced endocytosis is inhibited during mitosis. Live-cell confocal imaging experiment, mitotic cell expressing CD8–mCherry–FRB with FKBP–β2–GFP. Rapamycin (200 nM) was applied during metaphase. Clear rerouting occurs, but no puncta form in the cytoplasm until after cytokinesis. This cell is shown in Video 5. Bar, 10 µm.