Hot-wired endocytosis does not inhibit normal CME. (A) Confocal images of HeLa cells expressing FKBP–β2–GFP alone or together with CD8–mCherry–FRB were tested for ability to internalize transferrin–Alexa 647 (A647-Tf) in the presence or absence of rapamycin (200 nM; filled orange bar). Bars: (main) 10 µm; (inset) 2 µm. (B) Quantification of transferrin uptake and number of GFP-positive puncta in cells. Scatterplot shows values for each cell, bars indicate mean ± SD. Number of experiments = 2. Tf, ANOVA, P = 0.1; GFP, ANOVA, P = 5.89 × 10⁻⁶.