Figure 6.
Figure 6. Dynamics of Mff on ER. (A) Independent Mff punctae dynamics (Airyscan microscopy time-lapse). Left: Merged image of a live U2OS cell expressing ER-tagRFP (ER, red), GFP-Mff-S (green), eBFP2-Peroxisome (blue), and mPlum-mito3 (gray). Right: Time-lapse series of the inset, with independent Mff punctum associating with ER then transferring to mitochondrion (yellow arrow). Blue arrow, peroxisomally associated Mff; white arrow, mitochondrial Mff. Bars: (left) 2 µm; (inset) 1 µm. Time in seconds. See also Video 7. (B) Zoom of A, showing heterogeneous nature of peroxisomally associated Mff. Bars, 0.5 µm. (C) Dot plot showing diameter of peroxisomal Mff and independent Mff punctae from Airyscan images. 14 peroxisomal Mff (0.42 ± 0.050 µm) and 19 independent Mff punctae (0.22 ± 0.056) analyzed.

Dynamics of Mff on ER. (A) Independent Mff punctae dynamics (Airyscan microscopy time-lapse). Left: Merged image of a live U2OS cell expressing ER-tagRFP (ER, red), GFP-Mff-S (green), eBFP2-Peroxisome (blue), and mPlum-mito3 (gray). Right: Time-lapse series of the inset, with independent Mff punctum associating with ER then transferring to mitochondrion (yellow arrow). Blue arrow, peroxisomally associated Mff; white arrow, mitochondrial Mff. Bars: (left) 2 µm; (inset) 1 µm. Time in seconds. See also Video 7. (B) Zoom of A, showing heterogeneous nature of peroxisomally associated Mff. Bars, 0.5 µm. (C) Dot plot showing diameter of peroxisomal Mff and independent Mff punctae from Airyscan images. 14 peroxisomal Mff (0.42 ± 0.050 µm) and 19 independent Mff punctae (0.22 ± 0.056) analyzed.

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