Figure 9.
Figure 9. Reexpression of GFP-tagged SNX5 or SNX6 rescues the normal steady-state distribution of CI-MPR in the SNX5+6 KO c13 clonal line. (A) The SNX5+6 KO clonal line c13 was lentivirally transduced with GFP-SNX5, GFP-SNX6, GFP-SNX4, GFP-SNX8, or GFP alone. Colocalization analysis of endogenous CI-MPR and TGN marker TGN46 allowed comparison of CI-MPR distribution between the transduced lines and parental HeLa. Bars: (main images) 40 µm; (zooms) 20 µm. (A, right) n = 3 independent experiments; parental HeLa, 63 cells; +GFP, 70 cells; +SNX5, 72 cells; +SNX6, 78 cells; +SNX8, 66 cells; +SNX4, 73 cells (means ± SEM; one-way ANOVA compared with +GFP. ***, P < 0.001). (B) A schematic for how the SNX1/2–SNX5/6 complex coordinates sequence-dependent cargo recognition of the CI-MPR with the biogenesis of tubular-based cargo-enriched transport carriers.

Reexpression of GFP-tagged SNX5 or SNX6 rescues the normal steady-state distribution of CI-MPR in the SNX5+6 KO c13 clonal line. (A) The SNX5+6 KO clonal line c13 was lentivirally transduced with GFP-SNX5, GFP-SNX6, GFP-SNX4, GFP-SNX8, or GFP alone. Colocalization analysis of endogenous CI-MPR and TGN marker TGN46 allowed comparison of CI-MPR distribution between the transduced lines and parental HeLa. Bars: (main images) 40 µm; (zooms) 20 µm. (A, right) n = 3 independent experiments; parental HeLa, 63 cells; +GFP, 70 cells; +SNX5, 72 cells; +SNX6, 78 cells; +SNX8, 66 cells; +SNX4, 73 cells (means ± SEM; one-way ANOVA compared with +GFP. ***, P < 0.001). (B) A schematic for how the SNX1/2–SNX5/6 complex coordinates sequence-dependent cargo recognition of the CI-MPR with the biogenesis of tubular-based cargo-enriched transport carriers.

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