pac-1, picc-1, and cdc-42 regulate AJ protein level. (A–E) Quantitative intensity of fluorescently tagged AJ proteins in the indicated genetic background. Control embryos for RNAi experiments were treated with empty vector RNAi. “cdc-42(CA)” is epidermal overexpression of constitutively active HA-CDC-42. Intensity values were normalized such that the mean control value was 1. Individual data points from two independent experiments were pooled. (A) HMR-1-mCherry: control (n = 15) and pac-1(RNAi) (n = 15); (B) JAC-1-GFP: control (n = 15) and pac-1(RNAi) (n = 15); (C) DLG-1-GFP: control (n = 24) and pac-1(RNAi) (n = 21); (D) HMR-1-mCherry: control (n = 19) and picc-1(RNAi) (n = 19); (E) HMR-1-mCherry: control (n = 23) and cdc-42(CA) (n = 20). Red bars and error bars are mean ± SD. P-values were calculated using a Mann-Whitney U test. ***, P < 0.0001; NS, P > 0.05.