CDC-42, PAC-1, and PICC-1 localization at AJs. (A) Superficial view of HA-CDC-42 and HMR-1 coimmunostaining in the dorsal epidermis of a bean-stage embryo. (B) Internal view of mCherry-PAC-1 and HMR-1 coimmunostaining in a bean-stage embryo showing mCherry-PAC-1 colocalization with HMR-1. (C) Internal view of mCherry-PAC-1 and PICC-1-GFP costaining showing colocalization at junctions. (D) Internal view of mCherry-PAC-1 and GFP-CDC-42 in a live bean-stage embryo. The boxed region of epidermal cells is shown in the inset below. Bars, 5 µm.