Anaphase spindle rescue correlates with strong E-cadherin–mediated cell–cell adhesion. (A) Uninduced control (+Dox) and Par1b–overexpressing (−Dox) MDCK cells were fixed and stained for the adherens junctions proteins E-cadherin, p120 catenin, and β-catenin (see Fig. S2). The intensity of cortical E-cadherin was quantified. Shown are E-cadherin intensity spectrum maps. (Bottom) Enlarged areas of the rectangles in the top panels. Red arrowhead shows hepatocytic-like lateral lumina. (B) FRET efficiency of the E-cadherin tension sensor at cell–cell contacts in control (CAT) or Par1b–expressing MDCK-EcadTSMod cells. Depicted are examples of pre- and postbleach images and the corresponding intensity spectrum maps of the TFP-donor channel. (C–E) Uninduced control (+Dox) and ΔEcad (−Dox) MDCK cells were fixed and stained as indicated. (C) Asterisks indicate nonsubstrate-contacting cells. The percentage of cytokinesis with both daughter cells contacting the substratum (D) and the β angle in metaphase and anaphase profiles (E) were quantified. (D) Dotted lines and black arrowhead represent cell contours and daughter that lost substrate contact, respectively. (A, B, D, and E) n = 20–25 cells/experiment were analyzed for N = 3 independent experiments. Error bars indicate +SD (bar graphs) or ±SEM (dot graph). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ns, not significant, analyzed by t test.