Neural crest cell migration is decreased when DAN is overexpressed in the paraxial mesoderm. (A) Schematic representation of DAN protein/DiI injections in to the paraxial mesoderm. (A′) Schematic representation of the measurements made for analysis of DAN-related phenotypes. (B) HH St 15 embryo after injection of DAN protein into the paraxial mesoderm showing the injected side (top) and uninjected control side (bottom). Less neural crest (NC) cells migrate toward the branchial arch when DAN is overexpressed (asterisks). (C) Area of the branchial arch region containing neural crest cells 16 h after injection. n = 22 embryos. (C′) Distance of the branchial arch that neural crest cells reached 16 h after injection. n = 22 embryos. Two-sided Student’s t test. (D) Transverse section through an embryo in which DAN and DiI (magenta) have been injected into the paraxial mesoderm. (D′) Same section as in D, neural crest cells only. (E) Transverse section through an embryo in which Ringers and DiI (magenta) have been injected into the paraxial mesoderm. (E’) Same section as in E, neural crest cells only. Bars: (B) 100 µm; (D and E) 50 µm. NT, neural tube; OV, otic vesicle.