Neural crest and melanoma cells avoid DAN in vitro. (A) Images of a time lapse showing how a single cranial neural crest (NC) cell contacts DAN (red) and then changes the direction of migration. Bar, 15 µm. (B) Image from a time lapse of migrating cranial neural crest cells grown on alternating stripes containing DAN after 12 h of imaging. Bar, 50 µm. (C) Box plot of the percentage of neural crest cells on stripes either containing no factor (red, n = 10 cultures), DAN (black, n = 9 cultures), or DAN and BMP4 (blue, n = 3 cultures). (D) Images of a time lapse of a single melanoma cell (green) contacting and then migrating along the edge of the DAN (red). Bar, 30 µm. (E) Image from a time lapse of migrating melanoma cells grown on alternating stripes containing DAN after 12 h of imaging. Bar, 50 µm. (F) Box plot of the percentage of melanoma cells on stripes either containing no factor (red, n = 12 cultures), DAN (black, n = 18 cultures), or DAN and BMP4 (blue, n = 20 cultures). Two-sided Student’s t test.