Drp1 is recruited to Parkin foci and triggers fission of mitochondrial subdomains coated in Parkin. (A) Tet-ON: ΔOTC-expressing HeLa cells expressing YFP-Parkin (green) with or without a Drp1 KO background were treated with DOX for 48 h or for 48 h with a 24- or 48-h washout of DOX. Cells were fixed and stained for TOM20 (blue) and ΔOTC (red). (B) Tet-ON: ΔOTC-expressing HeLa cells expressing YFP-Parkin (green) with or without a Drp1 KO background with or without DOX treatment were fixed for 48 h and stained for Drp1 (red) and TOM20 (blue). Bars: (A and B, top) 10 µm; (B, bottom) 5 µm. (C) Quantification of the percentage of cells described in A with Parkin foci recruitment to mitochondria. n = 3. **, P < 0.01. Error bars indicate SD.