Figure 5.
Figure 5. Proteotoxic stress induces mitochondrial fission in a PINK1-dependent manner. (A) Tet ON: ΔOTC-expressing HeLa cells expressing YFP-Parkin (green) were treated with DOX for 48 h, stained for TOM20 (red), and imaged. Boxes mark cropped images shown below. (B) Tet ON: WT OTC or ΔOTC-expressing HeLa cells expressing YFP-Parkin (green) were treated with DOX for 72 h, stained for OTC (red) and TOM20 (blue), and imaged. (C) Tet ON: ΔOTC-expressing HeLa cells expressing YFP-Parkin (green) with or without treatment with DOX for 48 h and with or without a PINK1 KO background were stained for TOM20 (red) and imaged. Bars: (A, top, and B and C) 10 µm; (A, bottom) 5 µm. (D) Quantification of the percentage of cells with fragmented mitochondria that also do or do not display focal Parkin recruitment in the cells described in C. **, P < 0.01; ***, P < 0.001. Error bars indicate SD.

Proteotoxic stress induces mitochondrial fission in a PINK1-dependent manner. (A) Tet ON: ΔOTC-expressing HeLa cells expressing YFP-Parkin (green) were treated with DOX for 48 h, stained for TOM20 (red), and imaged. Boxes mark cropped images shown below. (B) Tet ON: WT OTC or ΔOTC-expressing HeLa cells expressing YFP-Parkin (green) were treated with DOX for 72 h, stained for OTC (red) and TOM20 (blue), and imaged. (C) Tet ON: ΔOTC-expressing HeLa cells expressing YFP-Parkin (green) with or without treatment with DOX for 48 h and with or without a PINK1 KO background were stained for TOM20 (red) and imaged. Bars: (A, top, and B and C) 10 µm; (A, bottom) 5 µm. (D) Quantification of the percentage of cells with fragmented mitochondria that also do or do not display focal Parkin recruitment in the cells described in C. **, P < 0.01; ***, P < 0.001. Error bars indicate SD.

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