JIP3 haploinsufficient 5xFAD animals do not exhibit defects in glial recruitment to plaques. (A–D) Representative images of 5xFAD (A) controls and JIP3+/−; 5xFAD (C) gender-matched littermate brain sections stained for LAMP1 (plaque; red) and Iba-1 (activated microglia; green. Bars, 10 µm. (B and D) Correlation between number of microglia at plaque with plaque size from 5xFAD (B) and JIP3+/−; 5xFAD (D) mice showing that the number of activated microglia at a plaque is similarly correlated with the plaque size in both genotypes (n = 3 pairs of gender-matched littermates; 30–40 plaques per animal per genotype analyzed). (E and F) Representative images from 5xFAD (E) and JIP3+/−; 5xFAD (F) cerebral cortices stained for GFAP (astrocytes; red) and LAMP1 (lysosomes; green) showing similar astrocyte recruitment to plaques in both genotypes. Bars, 10 µm. (G) Quantification of mean number of astrocytes per plaque (mean ± SD) in three pairs of 5xFAD controls and JIP3+/−; 5xFAD gender-matched littermates. 30–45 plaques and their associated astrocytes were analyzed per animal per genotype.