Figure 5.
Figure 5. Impact of JIP3 KO on the abundance and localization of BACE1. (A and B) Representative images of WT and JIP3 KO primary cortical neurons stained for BACE1 and LAMP1. BACE1 is seen in LAMP1-negative puncta in cell bodies of both genotypes, whereas JIP3 KO neurons also contain large amounts of BACE1 coaccumulating with LAMP1 in the axonal swellings (arrows, n = 5 independent JIP3 KO cultures). Bars, 10 µm. (C) Immunoblotting reveals increased levels of BACE1 protein in JIP3 KO neurons compared with WT neurons (VAPB used as loading control; n = 4; mean ± SD; ***, P < 0.001; unpaired t test). (D) WT and JIP3 KO primary axons (straightened) expressing APP and BACE proteins tagged with split Venus fragments (APP: VN+BACE-1: VC). Note the accumulation of the complemented (interacting proteins) at axonal swellings in JIP3 KO. Bar, 5 µm.

Impact of JIP3 KO on the abundance and localization of BACE1. (A and B) Representative images of WT and JIP3 KO primary cortical neurons stained for BACE1 and LAMP1. BACE1 is seen in LAMP1-negative puncta in cell bodies of both genotypes, whereas JIP3 KO neurons also contain large amounts of BACE1 coaccumulating with LAMP1 in the axonal swellings (arrows, n = 5 independent JIP3 KO cultures). Bars, 10 µm. (C) Immunoblotting reveals increased levels of BACE1 protein in JIP3 KO neurons compared with WT neurons (VAPB used as loading control; n = 4; mean ± SD; ***, P < 0.001; unpaired t test). (D) WT and JIP3 KO primary axons (straightened) expressing APP and BACE proteins tagged with split Venus fragments (APP: VN+BACE-1: VC). Note the accumulation of the complemented (interacting proteins) at axonal swellings in JIP3 KO. Bar, 5 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal