Figure 4.
Figure 4. Localizations of Vps27 variants during different growth phases. VPS27-deleted (vps27Δ) strains expressing Envy-tagged WT (A), FYVE-domain–mutated Vps27 (B), or clathrin-binding motif–deleted Vps27 (C) were cultured in YEPD medium at 28°C for 8 h (at an early log phase) or 14 h (after diauxic shift). Both cells labeled with FM 4–64 and nonlabeled cells were analyzed by fluorescence microscopy with the same image-acquisition parameters. The merge image consists of green Envy image and red FM 4–64 image. Bars, 2 µm. (D) The numbers of cells exhibiting diffuse, punctate, and/or rim patterns of Envy-Vps27 were counted (n > 50) for each observation, and the percentages of the patterns are represented in the stacked bar chart. Error bars indicate standard deviation.

Localizations of Vps27 variants during different growth phases. VPS27-deleted (vps27Δ) strains expressing Envy-tagged WT (A), FYVE-domain–mutated Vps27 (B), or clathrin-binding motif–deleted Vps27 (C) were cultured in YEPD medium at 28°C for 8 h (at an early log phase) or 14 h (after diauxic shift). Both cells labeled with FM 4–64 and nonlabeled cells were analyzed by fluorescence microscopy with the same image-acquisition parameters. The merge image consists of green Envy image and red FM 4–64 image. Bars, 2 µm. (D) The numbers of cells exhibiting diffuse, punctate, and/or rim patterns of Envy-Vps27 were counted (n > 50) for each observation, and the percentages of the patterns are represented in the stacked bar chart. Error bars indicate standard deviation.

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