Figure 2.
Immunoblot analyses detecting cleavage of Vph1-EGFP expressed in mutant strains defective in Atg pathways. The denoted ATG gene–deleted mutant strains (A) or other gene-deletion mutants (B) were cultured at 28°C for 24 h in YEPD medium and subjected to immunoblot analysis as shown in Fig. 1 A.