Reduction of GTSE1 levels reduces anaphase chromosome segregation defects in HeLa and U2OS cells. (A) Immunofluorescence images of anaphase U2OS cells after control, GTSE1, MCAK, or GTSE1 and MCAK siRNA stained for DNA (DAPI) and kinetochores (CREST). White arrows indicate anaphase segregation defects. (B) Quantification of the percentage of anaphase HeLa cells with defective anaphase chromosome segregation events. n > 390 per condition; 3 experiments. (C) Quantification of the percentage of anaphase U2OS cells with defective anaphase chromosome segregation events from immunofluorescence analysis as shown in A. The left graph shows U2OS cells after RNAi conditions as in A. n > 65 per experiment; 3 experiments per condition. The right graph shows two independent U2OS GTSE1 knockout clones. P-values were obtained from a χ2 test comparing against the U2OS control condition (marked with •). n > 100 cells. **, P ≤ 0.01.
