Figure 8.
Figure 8. Pex30p and Pex31p regulate the morphology of PPVs. (A) Immunogold EM images of pex3atg1Δ cells expressing endogenous Pex14-GFP using anti-GFP antibodies. (B) As in A, except with pex30pex31pex3atg1Δ cells. Examples of two types of PPV morphology shown: large vesicles (I) and clustered vesicles (II). (C) Quantification of diameter of nonclustered PPVs identified by anti-GFP immuno-EM on cells expressing Pex14-GFP using anti-GFP antibody (mean ± SE in 20 cells; ***, P < 0.005). (D) Immunogold EM images of pex3atg1Δ cells expressing endogenous Pex14-GFP and overexpressing Pex30p using anti-GFP and anti-Pex30p antibodies (red arrowheads, Pex14-GFP; yellow arrowheads, Pex30p). CW, cell wall; N, nucleus; P, preperoxisomal vesicle; V, vacuole.

Pex30p and Pex31p regulate the morphology of PPVs. (A) Immunogold EM images of pex3atg1Δ cells expressing endogenous Pex14-GFP using anti-GFP antibodies. (B) As in A, except with pex30pex31pex3atg1Δ cells. Examples of two types of PPV morphology shown: large vesicles (I) and clustered vesicles (II). (C) Quantification of diameter of nonclustered PPVs identified by anti-GFP immuno-EM on cells expressing Pex14-GFP using anti-GFP antibody (mean ± SE in 20 cells; ***, P < 0.005). (D) Immunogold EM images of pex3atg1Δ cells expressing endogenous Pex14-GFP and overexpressing Pex30p using anti-GFP and anti-Pex30p antibodies (red arrowheads, Pex14-GFP; yellow arrowheads, Pex30p). CW, cell wall; N, nucleus; P, preperoxisomal vesicle; V, vacuole.

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