Figure 7.
Figure 7. Pex30-2xmCherry is enriched at sites of PPV production. (A) Velocity of Pex14-GFP punctae; mean ± SE of 70 cells. *, P < 0.05. (B) Fluorescence microscopy images of pex3atg1Δ cells expressing endogenous Pex30-2xmCherry and either Pex14-YFP or Sec13-GFP. Stacks of three images with a step of size of 0.3 µm were taken and deconvolved; images from the indicated plane (z) are shown. (C) Quantification of experiment in B. n, number of Pex14-GFP or Sec13-GFP punctae. (D) Cells were grown as in Fig. 6 D, and stacks of three images with a step of size of 0.25 µm were taken at the indicated times and deconvolved. Images were used to quantify percent colocalization of GFP-Pex14 with Pex30-mCherry. n, number of GFP-Pex14 punctae counted. (E) Endogenous Pex14-GFP protein level of pex3atg1Δ cells grown in raffinose and protein level of GFP-Pex14 induced by galactose for 15 min and grown in raffinose for indicated time points.

Pex30-2xmCherry is enriched at sites of PPV production. (A) Velocity of Pex14-GFP punctae; mean ± SE of 70 cells. *, P < 0.05. (B) Fluorescence microscopy images of pex3atg1Δ cells expressing endogenous Pex30-2xmCherry and either Pex14-YFP or Sec13-GFP. Stacks of three images with a step of size of 0.3 µm were taken and deconvolved; images from the indicated plane (z) are shown. (C) Quantification of experiment in B. n, number of Pex14-GFP or Sec13-GFP punctae. (D) Cells were grown as in Fig. 6 D, and stacks of three images with a step of size of 0.25 µm were taken at the indicated times and deconvolved. Images were used to quantify percent colocalization of GFP-Pex14 with Pex30-mCherry. n, number of GFP-Pex14 punctae counted. (E) Endogenous Pex14-GFP protein level of pex3atg1Δ cells grown in raffinose and protein level of GFP-Pex14 induced by galactose for 15 min and grown in raffinose for indicated time points.

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