Pex30p and Pex31p play a role in PPV biogenesis. (A) Peroxisome biogenesis was induced as described in Materials and methods; graph shows percentage of cells containing mature peroxisomes at indicated time points after induction of Pex3p expression (n = 3). (B) Immunoblots of the strains in A with antibodies against Pex3p and anti-porin (as a loading control). (C) Mean number of Pex14-GFP puncta in the indicated strains; mean ± SE of three replicates, at least 100 punctae counted per replicate (*, P < 0.05; **, P < 0.005). (D) The results in C were regraphed to show the mean number of cells with the indicated number of Pex14-GFP punctae.