Figure 4. Pex30p localizes to subdomains... | Rockefeller University Press

Figure 4.

$Figure 4. Pex30p localizes to subdomains of the ER. (A) Fluorescence microscopy (FM) images expressing endogenous Pex30-2xmCherry and Sec63-GFP focusing on the center or periphery of cells. Yellow arrows indicate Pex30-GFP subdomains in ER tubules, and white arrows indicate Pex30-GFP at the edges of ER sheets. Bar, 3 µm. (B) Immunoblots of whole-cell extracts from the indicated strains; equal amounts of protein were separated using 4–12% SDS-PAGE. (C) FM images (top) of cells expressing endogenous Pex30-2xmCherry and Rtn1-GFP. The intensity of the pixels was measured using ImageJ. Bar, 3 µm. The line profile graph (bottom) shows the pixel intensities for Pex30-2xmCherry (red) and Rtn1-GFP (green) along the line drawn across the cell as shown in the figure. (D) Cell lysate were fractionated by centrifugation on a Histodenz step gradient. Pex30p cofractionated with the ER marker Dpm1p. Pex14-GFP was used as a marker for peroxisomes.$

Pex30p localizes to subdomains of the ER. (A) Fluorescence microscopy (FM) images expressing endogenous Pex30-2xmCherry and Sec63-GFP focusing on the center or periphery of cells. Yellow arrows indicate Pex30-GFP subdomains in ER tubules, and white arrows indicate Pex30-GFP at the edges of ER sheets. Bar, 3 µm. (B) Immunoblots of whole-cell extracts from the indicated strains; equal amounts of protein were separated using 4–12% SDS-PAGE. (C) FM images (top) of cells expressing endogenous Pex30-2xmCherry and Rtn1-GFP. The intensity of the pixels was measured using ImageJ. Bar, 3 µm. The line profile graph (bottom) shows the pixel intensities for Pex30-2xmCherry (red) and Rtn1-GFP (green) along the line drawn across the cell as shown in the figure. (D) Cell lysate were fractionated by centrifugation on a Histodenz step gradient. Pex30p cofractionated with the ER marker Dpm1p. Pex14-GFP was used as a marker for peroxisomes.

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