Figure 3.
Figure 3. Pex30p and Pex31p are reticulon like ER-shaping proteins. (A) Fluorescence microscopy (FM) images of rtn1rtn2yop1Δ cells expressing ss-RFP-HDEL and Pex30-GFP or Pex31-GFP from high-copy plasmids. Bar, 3 µm. (B) Predicted domain architecture of Pex30p; reticulon-like domain was identified using HHpred. The DysF domain has two parts, DysFN and DysFC. (C) Conserved tryptophan shown in red in the aligned amino acid sequence of Rtn1p, Rtn2p, Pex30p, and Pex31p using HHpred. (D) Serial dilutions of cultures of the indicated strains as in Fig. 1 B. (E) FM images of cortical ER in the indicated strains expressing Sec63-GFP. Bar, 3 µm. (F) Heterozygous spo7Δpex30Δpex31Δ diploid cells were sporulated, and the haploid spores were separated and grown on plates. Genotypes of selected haploids are indicated. 5-FOA, 5-fluoroorotic acid.

Pex30p and Pex31p are reticulon like ER-shaping proteins. (A) Fluorescence microscopy (FM) images of rtn1rtn2yop1Δ cells expressing ss-RFP-HDEL and Pex30-GFP or Pex31-GFP from high-copy plasmids. Bar, 3 µm. (B) Predicted domain architecture of Pex30p; reticulon-like domain was identified using HHpred. The DysF domain has two parts, DysFN and DysFC. (C) Conserved tryptophan shown in red in the aligned amino acid sequence of Rtn1p, Rtn2p, Pex30p, and Pex31p using HHpred. (D) Serial dilutions of cultures of the indicated strains as in Fig. 1 B. (E) FM images of cortical ER in the indicated strains expressing Sec63-GFP. Bar, 3 µm. (F) Heterozygous spo7Δpex30Δpex31Δ diploid cells were sporulated, and the haploid spores were separated and grown on plates. Genotypes of selected haploids are indicated. 5-FOA, 5-fluoroorotic acid.

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