Figure 4. Cofilin, but not F-actin... | Rockefeller University Press

Figure 4.

$Figure 4. Cofilin, but not F-actin disassembly, controls timely switching to phase 2. (A–F) Live wild-type (WT; black) and 1/2cofilin (turquoise) embryos. (G–L) Live DMSO (control; black) and phalloidin-injected (phalloidin; red) embryos. (A and G) Rings visualized with G-actinRed (A) or Sqh-GFP (G), constricting over time (minutes). Bars, 10 µm. (B and H) Ring perimeter versus time. (C and I) Constriction rate per phase. (D and J) Circularity 40 min after cellularization onset. (E and K) Switching time from phase 1 to phase 2. (F and L) t1/2 (F) or percent mobile fraction (L) for F-actin in photobleached rings (G-actinRed; F, n ≥ 26 rings from ≥13 embryos per genotype; L, n ≥ 7 rings from ≥7 embryos per condition; mean ± SE; see Fig. S1, E–H; and Table S1). (A, B, G, and H) Gray shading highlights phase 1 in wild-type embryos. (B and H) Arrowheads indicate switching time. (H) Arrow indicates injection time. (A) Wild-type images are the same as in Fig. 3 G and Fig. 5 (A and G). (B–E and H–K) n = 6 embryos per genotype or condition, five rings per embryo; mean ± SE. (C–F and I–L) *, P < 0.05; n.s., not significant.$

Cofilin, but not F-actin disassembly, controls timely switching to phase 2. (A–F) Live wild-type (WT; black) and 1/2cofilin (turquoise) embryos. (G–L) Live DMSO (control; black) and phalloidin-injected (phalloidin; red) embryos. (A and G) Rings visualized with G-actin^Red (A) or Sqh-GFP (G), constricting over time (minutes). Bars, 10 µm. (B and H) Ring perimeter versus time. (C and I) Constriction rate per phase. (D and J) Circularity 40 min after cellularization onset. (E and K) Switching time from phase 1 to phase 2. (F and L) t_1/2 (F) or percent mobile fraction (L) for F-actin in photobleached rings (G-actin^Red; F, n ≥ 26 rings from ≥13 embryos per genotype; L, n ≥ 7 rings from ≥7 embryos per condition; mean ± SE; see Fig. S1, E–H; and Table S1). (A, B, G, and H) Gray shading highlights phase 1 in wild-type embryos. (B and H) Arrowheads indicate switching time. (H) Arrow indicates injection time. (A) Wild-type images are the same as in Fig. 3 G and Fig. 5 (A and G). (B–E and H–K) n = 6 embryos per genotype or condition, five rings per embryo; mean ± SE. (C–F and I–L) *, P < 0.05; n.s., not significant.