Figure 2.
![Figure 2. Constriction phases correlate with distinct dynamics of ring components. (A–H) Fixed wild-type embryos. Gray shading highlights phase 1. (A, C, E, and G) Rings in cross section at increasing furrow lengths, stained for F-actin (phalloidin; A), Myosin-2 (C), Pnut (E), and Mbs (G). Arrowheads show ring position at furrow tips. Bars, 10 µm. (B, D, F, and H) Fluorescence intensity (arbitrary units [AU]) versus furrow length for F-actin (B), Myosin-2 (D), Pnut (F), and Mbs (H; n ≥ 43 embryos per staining and 10 ring profiles per embryo). Splines show trend of change for data points. Clouds show one SD from the moving mean.](https://cdn.rupress.org/rup/content_public/journal/jcb/215/3/10.1083_jcb.201608025/8/jcb_201608025_fig2.jpeg?Expires=1772730670&Signature=WEmB9LJMy~BXUJI9PaF-wItushZx9e1ppUBJJDCSa9fep8bFfIkHFA7i2dukydMYeNJMTa0zuMV12hBq3jUOlq1VGw65TWtzUu-d-0uaPsWxGZ5wS2mCnAiMM4YCWbwZxDsEX11BeLnyTcwtM7feNj8PyZqw5zfRuCR0KOGrOUFVpQLZWgQ4g~AuMcXbnN41~EQliEy6a0C412sibbKHnKgHyBYJYQWo2B-hhRvLJsD9kgIL1RXT7V6v8BvNvzgq0Ritc8--Ft~XkNZaifJwy5tyZ2rifEi8CuvdW~O0yqv9Cq~yrktVJl-iUTASCiJzQy4W6EwKJRp8cuv2P6a25g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Constriction phases correlate with distinct dynamics of ring components. (A–H) Fixed wild-type embryos. Gray shading highlights phase 1. (A, C, E, and G) Rings in cross section at increasing furrow lengths, stained for F-actin (phalloidin; A), Myosin-2 (C), Pnut (E), and Mbs (G). Arrowheads show ring position at furrow tips. Bars, 10 µm. (B, D, F, and H) Fluorescence intensity (arbitrary units [AU]) versus furrow length for F-actin (B), Myosin-2 (D), Pnut (F), and Mbs (H; n ≥ 43 embryos per staining and 10 ring profiles per embryo). Splines show trend of change for data points. Clouds show one SD from the moving mean.
