RGC translocation is stalled upon aPKC-CAAX overexpression and is not rescued over time. (A) No RGC translocation upon aPKC-CAAX overexpression. (B) Zn5 staining for differentiated RGCs in control retina at 48 hpf. Bar, 20 µm. (C) Zn5 staining of aPKC-CAAX–overexpressing retina at 48 hpf. (B and C) Dashed lines delimit apical and basal sides. (D) aPKC-CAAX expression specifically in the ath5 lineage stops RGC translocation and leads to ectopic polarization. (A and D) White dots, RGC followed; arrowheads, apical process; asterisk, loss of apical process; blue arrows, axon. Time is shown in hours and minutes. Bars, 10 µm. (E) The SoFa2 transgenic fish (combination of ath5:gap-RFP [labeling RGCs and photoreceptors (PRs)], crx:gap-CFP [labeling photoreceptors and bipolar cells (BCs)], and ptf1a:Gal4-VP16 UAS:gap-YFP [labeling horizontal cells (HCs) and ACs]) imaged every 12 h. Fish were kept in the incubator between time points. Ectopic RGCs developed at the temporal (T) side of retina. The nasal (N) side developed as a control (see Fig. S5 A). Arrowheads, clusters of RGCs that trigger ectopic lamination of other cell types; arrows, clusters of RGCs that interrupt the normal lamination without triggering ectopic layers; dashed boxes, magnified area. HS, heat shock; D, dorsal; V, ventral. Bars, 20 µm.