Basal process inheritance streamlines basal nuclear translocation in RGCs and progenitors. (A) Translocation of an RGC not inheriting the basal process (BP). Compare with Fig. 1 B. White dots, RGC followed; blue arrows, axon. Time is shown in hours and minutes. (B) Five representative 2D trajectories of RGCs inheriting (green) and RGCs not inheriting the basal process (BP; orange) for the first 95 min after cell division. More trajectories are in Fig. S2 (F and G). (C) Inheritance of basal process in progenitors. White dots, basal process inheriting progenitor; arrowheads, inherited basal process; arrows, newly formed basal process of sister cell. (A and C) Bars, 10 µm. (D) Five representative 2D trajectories of sister progenitors inheriting (blue) and not inheriting the basal process (gray) for the first 95 min after cell division. (E) Kinetics of RGC translocation with basal process. 0 indicates the mitotic position of cells. Single trajectories and a mean trajectory ± SD are shown. (F) Kinetics of RGC translocation without basal process. (E and F) Green phase, directionally persistent movement; gray phase, fine positioning. (G) MSDs of translocating RGCs with and without basal process. MSDs are calculated from the first 70 min after mitosis. (H) MSDs of faster and slower translocating sister progenitor nuclei. MSDs are calculated from the first 70 min after mitosis. (G and H) The α value is given with a 95% confidence interval. (I) Comparison of MSDs of RGC and progenitor nuclear translocation. Graph shows combined data from G and H. (J) Directionality ratios of RGC and progenitor nuclear translocation. The mean of all tracks is shown. Error bars represent SEM. Final directionality ratios: basal process RGC = 0.92, no basal process RGC = 0.82, fast progenitor = 0.75, slow progenitor = 0.49.