Inactivation of ZYG11A/B blocks mitotic slippage in nocodazole-treated cells. U2OS (A) and IMR90-hTERT (B) cells were treated with nocodazole under the conditions listed. Bar graphs represent the percentage of cells that underwent mitotic slippage or cell death. The χ² significance for the occurrence of mitotic slippage is relative to nocodazole + control siRNA. The graphs summarize the data from two to four independent experiments for each condition. From left to right: for A, n = 33, 39, 27, and 22; and for B, n = 17 and 25, respectively. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001.