DMD myotubes form highly aligned actin CSK but have less eccentric nuclear shape. (A) Representative images of D3 (i and ii) and D6 (iii and iv) healthy (i and iii) and DMD tissues (ii and iv) immunostained for f-actin (black) and nuclei (blue). Bars, 50 µm. (B) Actin OOP is plotted. n = 5 coverslips, 10 FOVs per sample, for each condition. (C) Nuclear OOP is plotted. n = 5 coverslips, 3 FOVs per sample, >400 nuclei analyzed for each day, for each condition. (D) Nuclear eccentricity is plotted. n = 5 coverslips, 3 FOVs per sample, >400 nuclei analyzed for each condition. For all data in this figure, *, P < 0.05 relative to other days within a condition; **, P < 0.05 relative to the other condition on the corresponding day. All FOVs were 318.5 × 318.5 µm. Error bars represent the mean ± SEM.