Figure 2.
Figure 2. DMD myoblasts exhibit decreased cytoskeletal and nuclear anisotropy. (A) Representative healthy (i) and DMD (ii) myoblast tissues immunostained for f-actin (black) and nuclei (blue). Bars, 50 µm. (B) Actin OOP is plotted. n = 5 coverslips, 10 FOVs per sample for each condition. (C) Myoblast nuclear OOP is plotted. (D) Myoblast nuclear eccentricity is plotted. (C and D) n = 5 coverslips, 3 FOVs per sample, >700 nuclei analyzed for each condition. (E) Schematic representations of healthy (i) and DMD (ii) myoblasts and a summary of results. Black lines represent actin fibrils. White arrows represent orientation of the nuclei (blue). For all data in this figure, **, P < 0.05. All FOVs were 318.5 × 318.5 µm. Error bars represent mean ± SEM.

DMD myoblasts exhibit decreased cytoskeletal and nuclear anisotropy. (A) Representative healthy (i) and DMD (ii) myoblast tissues immunostained for f-actin (black) and nuclei (blue). Bars, 50 µm. (B) Actin OOP is plotted. n = 5 coverslips, 10 FOVs per sample for each condition. (C) Myoblast nuclear OOP is plotted. (D) Myoblast nuclear eccentricity is plotted. (C and D) n = 5 coverslips, 3 FOVs per sample, >700 nuclei analyzed for each condition. (E) Schematic representations of healthy (i) and DMD (ii) myoblasts and a summary of results. Black lines represent actin fibrils. White arrows represent orientation of the nuclei (blue). For all data in this figure, **, P < 0.05. All FOVs were 318.5 × 318.5 µm. Error bars represent mean ± SEM.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal