Designing the tongue on a chip. (A) Schematic representation of tongue bending caused by contraction of a longitudinal skeletal muscle. (B) Schematic representation of the tongue on a chip. Immunostaining shows myotubes cultured on PDMS film in the magnified section (SAA, black; nuclei, blue). Bar, 50 µm. (C) Representative tissue culture images of myoblasts (i) and differentiated skeletal muscle (ii). Bars, 100 µm.