Figure 2.
Figure 2. The LINC complex is required for interphase NE rupture. (A) Western blot of U2OS GFP-NLS shLmnB1 lysates after transfection with indicated siRNAs. Antibodies used are listed at right. Protein levels are mean values from four Western blots. (B) Quantification of NE rupture after transfection of indicated siRNAs. Graph represents pooled data (columns) from three independent experiments (points, n > 100 cells). ns, P > 0.05; ***, P < 0.001, Fisher’s exact tests versus control. (C) Representative images of U2OS cells cotransfected with GFP-Nsp1 and mCherry-KASH1 or mCherry alone. Cells were pre-extracted with detergent before fixation in the bottom panels. (D) Quantification of NE rupture after transfection with indicated construct. Graph represents pooled data (columns) from three independent experiments (points, n > 75 cells). ***, P < 0.001, Fisher’s exact test. Bars, 10 µm.

The LINC complex is required for interphase NE rupture. (A) Western blot of U2OS GFP-NLS shLmnB1 lysates after transfection with indicated siRNAs. Antibodies used are listed at right. Protein levels are mean values from four Western blots. (B) Quantification of NE rupture after transfection of indicated siRNAs. Graph represents pooled data (columns) from three independent experiments (points, n > 100 cells). ns, P > 0.05; ***, P < 0.001, Fisher’s exact tests versus control. (C) Representative images of U2OS cells cotransfected with GFP-Nsp1 and mCherry-KASH1 or mCherry alone. Cells were pre-extracted with detergent before fixation in the bottom panels. (D) Quantification of NE rupture after transfection with indicated construct. Graph represents pooled data (columns) from three independent experiments (points, n > 75 cells). ***, P < 0.001, Fisher’s exact test. Bars, 10 µm.

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